One 250 ml can of Red Bull Energy Drink contains 80 mg of caffeine, about the same amount as in a cup of home-brewed coffee. The consumption of Red Bull Energy Drink should conform to a person's overall caffeine intake.

Many authorities around the world have studied the effects of caffeine. The European Food Safety Authority (EFSA) concluded in its 2015 scientific opinion on the safety of caffeine that an intake of up to 400 mg per day (equivalent to five 250 ml cans of Red Bull or five cups of coffee a day) does not raise safety concerns for healthy adults. This aligns with similar findings from the US Food and Drug Administration (FDA) and Health Canada. However, pregnant and nursing women should limit their caffeine intake to 200 mg per day.

All ingredients, including the caffeine content, are labeled on each can of Red Bull Energy Drink.